Characterization of the UGA-recoding and SECIS-binding activities of SECIS-binding protein 2
نویسندگان
چکیده
منابع مشابه
Characterization of the UGA-recoding and SECIS-binding activities of SECIS-binding protein 2
Selenium, a micronutrient, is primarily incorporated into human physiology as selenocysteine (Sec). The 25 Sec-containing proteins in humans are known as selenoproteins. Their synthesis depends on the translational recoding of the UGA stop codon to allow Sec insertion. This requires a stem-loop structure in the 3' untranslated region of eukaryotic mRNAs known as the Selenocysteine Insertion Seq...
متن کاملA short motif in Drosophila SECIS Binding Protein 2 provides differential binding affinity to SECIS RNA hairpins
Selenoproteins contain the amino acid selenocysteine which is encoded by a UGA Sec codon. Recoding UGA Sec requires a complex mechanism, comprising the cis-acting SECIS RNA hairpin in the 3'UTR of selenoprotein mRNAs, and trans-acting factors. Among these, the SECIS Binding Protein 2 (SBP2) is central to the mechanism. SBP2 has been so far functionally characterized only in rats and humans. In ...
متن کاملNovel structural determinants in human SECIS elements modulate the translational recoding of UGA as selenocysteine
The selenocysteine insertion sequence (SECIS) element directs the translational recoding of UGA as selenocysteine. In eukaryotes, the SECIS is located downstream of the UGA codon in the 3'-UTR of the selenoprotein mRNA. Despite poor sequence conservation, all SECIS elements form a similar stem-loop structure containing a putative kink-turn motif. We functionally characterized the 26 SECIS eleme...
متن کاملThe redox state of SECIS binding protein 2 controls its localization and selenocysteine incorporation function.
Selenoproteins are central controllers of cellular redox homeostasis. Incorporation of selenocysteine (Sec) into selenoproteins employs a unique mechanism to decode the UGA stop codon. The process requires the Sec insertion sequence (SECIS) element, tRNASec, and protein factors including the SECIS binding protein 2 (SBP2). Here, we report the characterization of motifs within SBP2 that regulate...
متن کاملFunctional characterization of the eukaryotic SECIS elements which direct selenocysteine insertion at UGA codons.
We investigated the requirements for selenocysteine insertion at single or multiple UGA codons in eukaryotic selenoproteins. Two functional SECIS elements were identified in the 3' untranslated region of the rat selenoprotein P mRNA, with predicted stem-loops and critical nucleotides similar to those in the SECIS elements in the type I iodothyronine 5' deiodinase (5'DI) and glutathione peroxida...
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ژورنال
عنوان ژورنال: RNA Biology
سال: 2014
ISSN: 1547-6286,1555-8584
DOI: 10.1080/15476286.2014.996472